Fig. 2. Cx32 protein is absent in Mist1^KO pancreas acinar cells at birth. (A-F) Mist1 heterozygous (Mist1^Het) and Mist1^KO pancreas samples were subjected to Cx32 immunofluorescence. Cx32-containing gap junctions (green, arrows) are absent in all Mist1^KO samples, regardless of age. Nuclei are stained with the DNA fluorochrome DAPI (blue). (G) Total RNA was isolated from wild type (WT) and Mist1^KO pancreas samples from postnatal day 1 (PN1), 3-week old, 4-month and 8-month-old animals and then subjected to RNA blot analysis using a mouse Cx32 probe. In all cases, Cx32 mRNA levels are significantly lower in the Mist1^KO samples than in the WT control mice. 12S rRNA hybridization was used as an internal control.