Fig. 2. Localization of HP1 subtypes in metaphase chromosome spreads. HP1
(a,b), HP1ß (c,d) and HP1
(e,f) are shown in green on metaphase
chromosomes (red). Metaphase chromosome spreads were prepared from cells
collected with (a,c,e) or without (b,d,f) enrichment of mitotic cells (see
Materials and Methods). Mitotic cells were about 22-24% of the total cell
population in enriched preparation and about 6-9% in the non-enriched
prerapation. Scale bar, 10 µm.