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Fig. 2. Localization of HP1 subtypes in metaphase chromosome spreads. HP1{alpha} (a,b), HP1ß (c,d) and HP1{gamma} (e,f) are shown in green on metaphase chromosomes (red). Metaphase chromosome spreads were prepared from cells collected with (a,c,e) or without (b,d,f) enrichment of mitotic cells (see Materials and Methods). Mitotic cells were about 22-24% of the total cell population in enriched preparation and about 6-9% in the non-enriched prerapation. Scale bar, 10 µm.