Fig. 3. Domains of HP1 required for its localization. HeLa cells transiently expressing HP1 fragments fused with YFP and the CENP-B N-terminal region fused with CFP. (A) Diagram of YFP-HP1 truncation constructs and their intracellular localization: +, localized; –, not localized; +/–, ambiguous; and ND, not determined. Quantitative data were obtained from three independent experiments. (B) YFP-HP1 fragment localization during metaphase: YFP-HP1 fragment (left column), CFP-CENPB-N-terminal fragment (middle column), and merged images (right column). (C) Localization of HP1 fragments in the interphase nucleus. (D) Localization of HP1 and PML nuclear bodies. Numbers on the left of each row refer to the amino acid numbers of the fragments. Scale bar, 10 µm.