Fig. 4. Domains of HP1ß required for its localization. HeLa cells transiently expressing HP1ß fragments fused with YFP and the CENP-B N-terminal region fused with CFP. (A) Diagram of YFP-HP1ß truncation constructs and their intracellular localization: +, localized; –, not localized; +/–, ambiguous; ND, not determined. Quantitative data were obtained from three independent experiments. (B) YFP-HP1ß fragment localization during metaphase: YFP-HP1ß fragment (left column), CFP-CENPB-N-terminal fragment (middle column), and merged images (right column). (C) Localization of HP1ß fragments in the interphase nucleus. (D) Localization of HP1ß and PML nuclear bodies. Numbers on the left of each row refer to the amino acid numbers of the fragments. Scale bar, 10 µm.