Fig. 5. Domains of HP1 required for its localization. HeLa cells transiently expressing HP1 fragments fused with YFP and the CENP-B N-terminal region fused with CFP. (A) Diagram of YFP- HP1 truncation constructs and their intracellular localization: +, localized; –, not localized; +/–, ambiguous; and ND, not determined. Quantitative data were obtained from three independent experiments. (B) YFP- HP1 fragment localization during metaphase: YFP- HP1 fragment (left column), CFP-CENPB-N-terminal fragment (middle column), and merged images (right column). (C) Localization of HP1 fragments in the interphase nucleus. (D) Localization of HP1 and PML nuclear bodies. Scale bar, 10 µm.