JCS -- Kinoshita et al. 116 (16): 3339 Figure IG1

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Fig. 1. Reagents used in this study. This scheme shows the constructs and antibodies used in this study. Membrane-spanning regions are shown in black. All APP constructs are tagged at the C-terminus with myc, V5 or EGFP; wild-type APP770, APP with Swedish mutant, APP delta49 which lacks the entire cytoplasmic domain. BACE constructs are either N-terminally tagged (with myc) or C-terminally tagged with V5. For labeling the ectodomain regions of APP and BACE, 8E5 and anti-myc antibody are used, respectively. For amyloid-ß ELISA, biotinylated 6E10 and BA27 are used to capture amyloid-ß.