Fig. 1. Nuf2 associates tightly with chicken Hec1 throughout the cell cycle. (A) Immunoprecipitation with pre-immune IgG or an anti-GFP antibody from extracts of Nuf39-15 cells in which Nuf2 was replaced with Nuf2-GFP and from Hec25-1/5 cells in which Hec1 was replaced with Hec1-GFP. Total extract (input) and the immune complexes (anti-GFP) were analyzed by immunoblotting with anti-Hec1 or anti-Nuf2 antibodies. (B) Localization of Nuf2-GFP (green) and Hec1-HcRed (red) at progressive stages of the cell cycle in DT40 cells that expressed Nuf2-GFP and Hec1-HcRed simultaneously. Nuclei and chromosomes are counterstained with DAPI (blue). The scale bars correspond to 10 µm. As shown in the merged images, Nuf2-GFP signals are colocalized with Hec1-HcRed signals throughout the cell cycle.