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Fig. 8. Nuf2 and Hec1 localization in CENP-H- and CENP-I-deficient cells. (A) Localization of Nuf2-GFP and Hec1-GFP in #5-5 ({Delta}/CENP-H, CENP-H transgene) cells grown in the absence (–tet) or presence (+tet) of tet for 48 hours. Hec1-GFP (green) or Nuf2-GFP (green) signals are reduced after addition of tet (+tet). (B) Localization analysis of Nuf2-GFP and Hec1-GFP in M6-90 ({Delta}/CENP-I, CENP-I transgene) cells grown in the absence (–tet) or presence (+tet) of tet for 48 hours. Hec1-GFP (green) or Nuf2-GFP (green) signals are reduced after addition of tet (+tet).