Fig. 9. Turnover of Nuf2-GFP and Hec1-GFP at centrosomes and centromeres. (A) The centrosomes and centromeres (white boxes) were targeted for laser photobleaching and then followed by fluorescence time-lapse microscopy. Pre-bleach, post-bleach, and recovery images are shown. See Movies 4-7 (http://jcs.biologists.org/supplemental). (B) Graphs illustrating the ratio of relative fluorescence recovery. For centrosome analysis, we used Origin software (OriginLab Corp.) to determine recovery half-times.