JCS -- Guha et al. 116 (16): 3373 Figure IG5

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Fig. 5. GFP-GPI is internalized into LR-Dex early endosomes and Cy5mBSA early endosomes. Hemocytes from Collagen-Gal4;UAS-GFP-GPI (A-D) were incubated with Fl-anti-GFP (pseudo-colored green; Cy5-anti-GFP in A,C and Alexa568-anti-GFP in B,D) at room temperature for the indicated times in the presence of LR-Dex (A,C; red) or Cy5mBSA (B,D, red). Note that Fl-anti-GFP is partially colocalized with LR-Dex (A, arrowheads; C, arrows; insets) and with Cy5mBSA (B, small arrows; D, arrows; insets) at these times. However, at late times, all three probes colocalize in late endosomes (arrows, C,D). Insets (red, top; green, middle; merge, bottom) show the area marked by the asterisk. Bar, 5 µm; inset, 1 µm.