Fig. 9. ALBR is localized to GFP-clathrin light chain marked sites at the plasma membrane. Hemocytes from Collagen-Gal4;UAS-GFP-Clc (A-C) or shi^ts2;Collagen-Gal4;UAS-GFP-Clc animals (D-F) were incubated with Cy3mBSA (A,D; red in C,F) on ice (0°C; A-C) or at a restrictive temperature (31°C; D-F) for 5 minutes prior to fixation, permeabilization and imaging on wide-field microscope. On ice, the peripherally distributed Cy3mBSA-positive structures (A, red in C; small arrowheads) are positive for GFP-Clc (B, green in C; small arrowheads). At 31°C in cells from shi^ts2 animals, Cy3mBSA remains peripherally distributed in punctate (D, small arrowheads) structures also positive for GFP-Clc (E, small arrowheads). This distribution in shi^ts2 cells at 31°C is similar to cells labeled with Cy3mBSA on ice (small arrowheads). Insets show magnified view of the area marked by an asterisk. Bar, 5 µm; inset, 1 µm.