Fig. 7. Regulation of gene expression during early development of cells overexpressing superoxide dismutase. SOD-OE and Ax2 cells were separately developed in shaking suspension at 2x10⁷ cells/ml and aliquots taken for RNA extraction at the indicated times after starvation. The RNA was blotted onto a nylon membrane, and probed with ³²P-labelled fragments of the indicated genes. carA, cAMP receptor cAR1; pdsA, extracellular phosphodiesterase; dscA, discoidin 1; acaA, aggregation stage adenylyl cyclase A; pkaC, the catalytic subunit of cAMP dependent protein kinase; IG7, loading control.