Fig. 5. Centriole splitting requires Eg5 activity. (A) Examples of MA-arrested mitoses from NIH/3T3 cultures transfected with GFP vector or with RanBP1-GFP and stained with antibody to -tubulin (right): no obvious difference in the centrosomal pattern is observed. Chromosomes are stained with DAPI and merged pictures are shown on the left. Scale bar, 10 µm. (B) Patterns of centrioles in monoastral mitoses revealed by anti-centrin-2 antibody: (a) normal arrangement with two pairs of centrioles; (b) supernumerary centriole pairs; (c) split centrioles. The rightmost column shows a magnification of the centrin 2 panels. Scale bar, 10 µm. (C) Frequency of centrosomal arrangements in NIH/3T3 MA-arrested and MA-released mitoses transfected with vector or RanBP1-GFP. Pooled data from four experiments were analysed using the ² test. *, P<0.01.