Fig. 5. Filament formation depends on SLT2. (A) Appearance of slt2 cells incubated for 16 hours in YEPD or YEPD plus 0.5% IAA. Scale bar: 10 µm. (B) Samples of wild-type cultures in the log-phase of growth were taken at the indicated times after addition of 0.5% IAA. Total protein extracts were probed by western blotting with anti-active (p44/42) Slt2 antisera. Values on the ordinate are the ratio of each experimental signal to its loading control.