Fig. 3. (A) Minimal Gag sequence requirement for MTOC targeting. Schematic representation of the GFP-Gag fusion proteins. The locations of the three glycine/arginine rich motifs (GR I, II, III), the Gag-Gag interaction domain (ID) and the cytoplasmic targeting and retention signal (CTRS) are indicated by black boxes. The numbers indicate the percentage of GFP-positive cells harboring a centrosomal staining; values are the average of three independent experiments. (B) Western-blot analysis of the different GFP fusions revealed by rabbit anti-GFP antibodies. (C) Subcellular localization of GFP-Gag fusions as observed by fluorescence microscopy and prediction of coiled-coil motifs in the first 270 amino acids of HFV Gag as revealed by the COILS program (matrix: MTIDK, weight of 2.5 for position N).