Fig. 1. Immunofluorescence analysis of connexins in cryosections of wild-type epidermis. Cx31 immunoreactivity was mainly detected in the granulous layer and the upper part of the spinous layer (A). In 20% of all specimen analyzed, Cx30 (B) and Cx26 (D) were weakly expressed in the granulous layer. Cx43 expression occurred in the basal as well as in the spinous layer (C). Nuclei were stained with Hoechst 33258 fluorescent dye. The basal lamina is marked by white dots. Bar, 25 µm. Epidermal layers: bs, basal; co, cornified; gr, granulous; sp, spinous.