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Fig. 4. Immunofluorescence labelling of Cx30 in cryosections of 4-hydroxytamoxifen-induced Cx43Cre-ER(T)/fl mouse tail epidermis (A) showed prominent immunoreactivity of Cx30 in the granulous and spinous layers, in contrast to barely visible Cx30 immuno signals in wild-type epidermis (B). Nuclei were stained with Hoechst 33258 fluorescent dye. The basal lamina is marked by white dots. Bars, 25 µm. Epidermal layers: bs, basal; co, cornified; gr, granulous; sp, spinous.