Fig. 8. ROCK-mediated signal is required for processing of procaspases-8 and -10 in PMA-treated TF-1 cells. TF-1 cells were either left untreated or pre-incubated with specific ROCK inhibitor (Y27632; 20 µM), Latrunculin B (L.B, 0.5 µM) or MLCK inhibitor (ML-9; 20 µM), as indicated. Eight hours after PMA treatment, cells were harvested and analyzed by immunoblotting with antibodies specific for caspase-3, -8 and -10. NT, not treated.