Fig. 2. The effect of Rab6A-T27N on the sensitivity of cells to Shiga toxin and ricin. Vero cells were either mock transfected () or transfected with a plasmid encoding mutant Rab6A-T27N () for 18 hours and treated with various concentrations of Shiga toxin (A) or ricin (B) for 2.5 hours. Tran ³⁵S-label was added for 30 minutes and the incorporation of radioactivity into acid-insoluble material was determined as described in Materials and Methods. Each point is the average of three independent experiments.