Fig. 4. The distribution of TGN-38 and Rab6A-GFP in ldlF cells. Cells were transfected with plasmid DNA encoding Rab6A-GFP and incubated for 48 hours at 34°C. Cells were left at 34°C (A and B) or shifted to 39.5°C for 6 hours (C and D). The cells were then fixed and stained with anti-TGN-38 followed by a rhodamine-labeled secondary antibody. (A) and (C) show rhodamine-labeled anti-TGN-38 and B and D are direct visualization of GFP in the fields shown in A and C, respectively. The arrows in C and D show examples of structures that label with both TGN-38 and Rab6A-GFP in ldlF cells at the high temperature. Scale bar in D: 25 µm.