Fig. 3. ECM enhances GJIC. Microinjection (A,C) and scrape loading (B,D) of LY in CID-9 cells on day 6 of culture. Cells were plated in differentiation medium on plastic (A,B) or on EHS-drip (C,D). The bar corresponds to about 2 cells in (A) and about 10 cells in (C). Integrated fluorescence intensity, quantified from three different LY scrape-load experiments as described in Materials and Methods, showed a threefold decrease between cells on EHS-drip (Drip) and on plastic (Pl).