Fig. 7. cAMP induces ß-casein expression in an adhesion-independent manner. (A) Morphology of CID-9 cells on day 6 of culture, plated on PolyHEMA (Ctrl; control) and supplemented with 50 µM 8-Br-cAMP (cAMP). (B) Western blot for ß-casein, Cx43, Cx32 and Cx26 and for ß-actin for cells plated on PolyHEMA (Ctrl; control) and supplemented with 50 µM 8-Br-cAMP (cAMP). Significant increase in ß-casein expression (P<0.001) and Cx43 (P<0.001) was noted when cells on polyHEMA were treated with 8-Br-cAMP compared with untreated cells.