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Fig. 6. Activation of Rac1 and suppression of RhoA through {alpha}6ß4 integrin control the localization and activation of {alpha}3ß1 integrin. (A) Effect of RhoA inhibition or Rac1 activation on {alpha}3ß1 distribution. ß4(AD) cells were transduced with control LacZ (top row), inhibitory N19RhoA (middle row) or activated V12Rac1 (bottom row) and examined by immunofluorescence microscopy. Left panel shows actin distribution with TRITC-phalloidin, center and right panels show {alpha}3 integrin and conformationally active ß1 integrin. (B) Effect of Rac1 inhibition on distribution and activation of {alpha}3ß1 integrin. ß4(+) cells were retrovirally transduced with control LacZ (upper row) or inhibitory N17Rac1 (lower row) and studied by immunofluorescence microscopy. Left panel shows {alpha}3 integrin, center panel shows paxillin and right panel shows distribution of conformationally active ß1 integrin. Scale bar: 20 µm.