Fig. 7. The localization of HSF1 and HSF2 in stress granules is suppressed in thermotolerant HeLa cells. (A) Fluorescence micrographs of the localization of HSF1 and HSF2 in untreated (C), heat-shocked (30' or 1h), thermotolerant (HS+3R) and heat-shocked thermotolerant (HS+3R+HS30' or HS+3R+HS1h) cells. Proteins were visualized using monoclonal antibodies against HSF1 (red) and polyclonal antibodies against HSF2 (green). Co-localization is shown in the merge lane (yellow) and DNA is stained with DAPI (blue). Scale bars, 5 µm. (B) The graph represents the means ± s.e.m. of the three independent experiments in which the number of granule-positive cells was analyzed by counting nine groups of 50 cells.