Fig. 1. Role of VE-cadherin in cell spreading and proliferation. (A,B) Phase contrast images of bovine pulmonary artery endothelial cells treated for 96 hours with anti-VE-cadherin (A) and control (B) antibodies. Scale bar: 50 µm. (C) Graph of mean projected area of endothelial cells with time after treatment with anti-VE-cadherin and control antibodies. (D) Graph of number of cells after treatment with anti-VE-cadherin and control antibodies, normalized to number of cells at 24 hours after plating. (E) Flow cytometry diagrams of cell-cycle distribution of unsynchronized cells (left) and synchronized cells (right). Quantification of cell-cycle distribution is shown for each condition. (F) Graph of percentage of cells in S phase (BrdU incorporation) and mitotic index with time after replating of synchronized cells. (G) Graph of mean projected area of endothelial cells as a function of seeding density after treatment with anti-VE-cadherin and control antibodies. (H) Graph of percentage of endothelial cells entering S phase (incorporating BrdU) as a function of seeding density after treatment with anti-VE-cadherin and control antibodies. Error bars represent the s.d., with (*) P<0.05 relative to controls as calculated by t-test.