Fig. 1. Expression of NDST-1 and -2 transcripts by different cell types. Samples were run in a 1.5% agarose gel; for each sample 100 µg of total RNA (A), or 1 µg of poly(A)⁺ RNA (B) was run and blotted onto a nylon membrane. RNA was isolated from the cell types displayed at the top of the figure. Northern blot analysis was performed using a random prime synthesised probe using [³²P]dCTP for NDST-1 (A) and an antisense probe labelled with [³²P]UTP for NDST-2 (B). The bands showing 18S rRNA within the total RNA indicate the relative loading of each of the samples. The size of the RNA species was determined by co-electrophoresis of mRNA size standards (Promega).