Fig. 3. Phenotypic analysis of the abundance of sulphated epitopes in HS. HMEC-1 cells were analysed by flow cytometry to measure the level of sulphation at 0, 16, 24, 48 and 72 hours after stimulation with TNF- and IFN-. (A) Representative flow cytometer fluorescence histograms showing the isotype control value (black) and data from time 0 (blue), 16 (green) and 72 hours (red); median values are also shown. (B) Summary results showing the increase in fluorescence due to binding of 10E4 antibodies to cytokine activated HMEC-1 cells; the dotted line indicates the upper level of isotype control fluorescence. Data points show the mean from triplicate determinations; the error bars indicate the s.e.m.