Fig. 5. Analysis of proteoglycan core protein expression by western blotting. Lysates from HMEC-1 cells stimulated for 4 hours or 16 hours with TNF- and IFN- were compared with those from unstimulated cells. All lysates were digested in the presence of chondroitinase ABC and heparatinase. The cleavage products were separated by SDS-PAGE, electrotransferred to hybond-P membranes and probed by mAb 3G10. The figure is a composite generated from gels containing 8% (A) and 12% (B) acrylamide, as indicated. Blots were reprobed with an anti (a)-tubulin antibody to provide an internal protein loading control (C). Apparent molecular masses of the core proteins are indicated.