Fig. 9. dTOR is required for dRheb-mediated growth. Time of eclosion (hatching from pupal case) in days after egg lay (AEL) is compared for siblings carrying either one (dRheb/+) or two (+/TM3) wild-type copies of dRheb in the presence or absence of 1 µM rapamycin; the proportions of the total eclosed for each genotype and growth condition are indicated (A). Mean eclosion time of 314 +/TM3 and 202 dRheb/+ sibs in medium without rapamycin was 9.6±0.8 days and 9.2±0.6 days, respectively, whereas the mean eclosion time of 190 +/TM3 and 126 dRheb/+ sibs in medium with rapamycin was 12.6±1.5 and 13.6±1.7 days, respectively. Treatment of S2 cells with rapamycin results in a decrease in cell size, as determined by forward scatter analysis (B). Although overexpression of dRheb leads to an increase in cell size, treatment of cells overexpressing dRheb with rapamycin inhibits dRheb-mediated cell growth (C).