Fig. 1. Rapid accumulation of PtdIns(3,4,5)P₃/PtdIns (3,4)P₂ at the T. cruzi invasion site. Fluorescence images of (A) CHO cells or (B) primary rat cardiomyocytes transiently expressing Akt-PH-GFP following incubation with infective T. cruzi trypomastigotes for 15 minutes. (C) T. cruzi invasion of CHO cells expressing Akt-PHGFP^R25C which fails to bind to PtdIns(3,4,5)P₃/PtdIns(3,4)P₂. Extracellular parasites were stained with T. cruzi-specific antibodies in non-permeabilized cells. Host cell and parasite DNA were visualized with DAPI (A-C, blue). (D) Recruitment of Akt-PH-GFP to membranes surrounding invading T. cruzi trypomastigotes is sensitive to wortmannin. The relative number of Akt-PH-GFP-associated parasites was determined in CHO cells infected for 15 minutes following treatment with 20 nM wortmannin or vehicle control for 30 minutes. Data are represented as means ± s.d. n=3.