Fig. 3. Lamp-1 association with the Akt-PH-GFP-enriched T. cruzi vacuole occurs after parasite entry. (A,B) Immunofluorescence staining of lamp-1 (red) in T. cruzi-infected CHO cells at 15 minutes post-infection showing diffuse (arrows) and punctuate (arrowheads) staining patterns outlining intracellular parasites. External parasites stained with T. cruzi-specific antibodies (green); parasite and mammalian DNA stained with DAPI (blue). (C-F) L6E9 myoblasts expressing Akt-PH-GFP (green), were infected with T. cruzi for 15 minutes (C,D) or 60 minutes (E,F) and stained with anti-lamp-1 (red) and DAPI (blue). No overlap of Akt-PH-GFP and lamp-1 staining is observed (C-F, merge).