Fig. 7. T. cruzi vacuole maturation involves an early PI 3-kinase-dependent step. CHO cells were pulsed with (A,B) T. cruzi or (C) IgG-coated latex beads for 10 minutes, washed extensively and incubated in the absence or presence of 40 nM wortmannin for a total of 10, 20, 30, 45 and 60 minutes. (D) Kinetics of lamp-1 association with the T. cruzi vacuole in untreated CHO cells (control), cells pre-treated with 40 nM wortmannin for 30 minutes (wortmannin-pre) or in wortmannin pretreated cells to which drug was added after 10 minutes of infection (wortmannin pre+post). Data are represented as means ± s.d.; n=3.