Fig. 3. Removal of the C-terminal domain abolishes most of the interactions of Pex12p. Cells containing Pex12C-C_ub-RUra3p and expressing the N_ub constructs of Pex3p, Pex4p, Pex10p, Pex11p, Pex12p, Pex13p, Pex14p, Pex15p, Pex19p and Pex22p were diluted to an OD₆₀₀ of 1, and 4 µl of this and of tenfold serial dilutions were spotted onto media containing 100 µM CuSO₄ and either lacking uracil (A) or containing both uracil and 5-FOA (B). Both media were lacking tryptophan and histidine to select for the plasmids. Cells were incubated at 30°C for 48 hours.