Fig. 7. Analysis of peroxin interactions in the absence of peroxisomal membranes. pex3 cells (A,B,E) containing Pex12-C_ub-RUra3p (A), Pex10-C_ub-RUra3p (B) or Pex11-C_ub-RUra3p (E) and expressing the indicated N_ub-fusions or the empty plasmid were diluted to an OD₆₀₀ of 1, and 4 µl of this and of tenfold serial dilution were spotted onto media containing 100 µM CuSO₄ and 5-FOA. The media lacked tryptophan and histidine to select for the plasmids. The cells were incubated at 30°C for 48 hours. (C) and (D) as in (E) but with wild-type cells (C) or pex11 cells (D).