Fig. 6. Disruption and intracellular redistribution of ZO-1 coincide with increased levels of ErbB2 in MCF-7B cells. (A) Cultures were established on glass coverslips, formaldehyde-fixed, CSK permeabilized and processed for IF staining with ErbB2 and ZO-1 antibodies. Scale bar: 50 µm. (B) TCEs were prepared for confluent MCF-7, MCF-7B and SKBR-3 cultures and 50 µg from each line processed for IB with ErbB2 antibodies.