Fig. 7. Bcl-2 expressed in MDCK cells inhibits junction formation and co-distributes with the soluble ZO-1. (A) Ultrastructural organization of uninduced and Zn²⁺-induced Bcl-2-expressing MDCK cells. MDCK-Bcl-2 transfectants were grown on collagen- and carbon-coated glass coverslips without (left) or with (right) Zn²⁺ and processed for electron microscopy. (B) Confluent Zn²⁺-induced, MDCK-Bcl-2 transfectants grown on coverslips were processed for double IF with a mixture of Bcl-2 and ZO-1 antibodies. Primary antibodies were detected with species-specific secondary antibodies and viewed with a 63x objective. (C) Duplicate cultures from B were either formaldehyde fixed, CSK-permeabilized (Fixed) or CSK extracted first and then fixed (Extracted) and processed for Bcl-2 and ZO-1 staining. (D) Duplicate cultures from B were formaldehyde fixed, CSK-permeabilized and processed for double IF staining with Bcl-2 and p120 antibodies. Scale bars: 1.25 µm (A); 85 µm (B); 40 µm (C); 50 µm (D).