Fig. 1. Distribution of B-type lamins, lamin A/C, NuMA and AKAP95 in mouse cumulus cells and in fertilized preimplantation embryos cultured in vitro. Immunofluorescence analysis of (A) cumulus cells and (B) mouse embryos at the pronuclear, 2-cell, 8-16-cell and blastocyst stages. (A inset) DNA. Scale bar, 10 µm. Arrow points to anti-AKAP95 labeling in the female pronucleus. (B inset) DNA labeled with Hoechst 33342. Scale bar, 50 µm. (C) MII oocytes and pronuclear stage fertilized embryos (PN) were triple-labeled using anti-AKAP95 polyclonal antibodies, anti-NuMA mAbs and Hoechst 33342 to visualize association of AKAP95 and NuMA with the female pronucleus (FPN). MPN, male pronucleus. Scale bar, 50 µm. (D) Immunoblotting analysis of cumulus cells and fertilized pronuclear stage embryos using antibodies against indicated proteins. Apparent M_r is shown in kDa.