Fig. 2. Dynamics of B-type lamins, lamins A/C, NuMA and AKAP95 following NT. Disassembly of the donor nucleus and reformation of the new nucleus were monitored at the premature chromatin condensation [PCC; 3 hours post-infection (hpi)] and interphase (NT Int.; 7 hpi) stages, using antibodies against indicated proteins. Female pronuclei formed after parthenogenetic activation of MII oocytes were also analysed 6 hours after start of activation treatment (Part PN). TRITC refers to labeling with TRITC-conjugated secondary antibodies. Scale bars, 20 µm. n>20 embryos per treatment.