Fig. 5. Passage through first mitosis does not restore abnormalities in lamin A/C labeling and intranuclear anchoring of AKAP95 in NT embryos. (A) Two-cell stage ICSI and NT embryos were analysed by immunofluorescence using anti-lamin-A/C mAbs. (B) Two-cell-stage ICSI and NT embryos were extracted 0.1% Triton X-100, 1 mg ml^-1 DNAse I, 100 µg m^-1 RNase A, 300 mM NaCl prior to immunofluorescence analysis using anti-AKAP95 antibodies. DNA was labeled with propidium iodide. Scale bars, 20 µm. (C) Relative DNA and AKAP95 immunolabeling intensities in intact (100%) and extracted two-cell-stage ICSI and NT embryos. n>10 embryos per treatment.