Fig. 1. Keratinocyte E-lam formation in mucosal wounds and in vitro. (A) Electron micrograph of migrating keratinocytes in 3-day-old human mucosal wounds. Arrows mark cellular extensions formed by wound keratinocytes. wb, wound bed; kc, keratinocyte. (B) A photograph of primary mouse keratinocytes grown in medium containing fibroblast conditioned medium and EGF (4 ng/ml). Arrows mark E-lams. (C) HaCaT keratinocytes were treated with EGF (4 ng/ml) in DMEM containing 1% FBS for 48 hours and then photographed. Arrows mark E-lams. Scale bar: 10 µm.