Fig. 10. Effect of GSK-3 inhibitors on cell migration. Confluent cell layers were scratch-wounded and then treated with staurosporine (50 nM; B,E,H), EGF (10 ng/ml; C,F,I) or left untreated (A,D,G) together with GSK-3 inhibitors SB-415286 (30 µM; D-F) and LiCl₂ (30 mM; G-I) for 24 hours. In EGF-treated samples, the wounded cultures were preincubated for two hours before adding EGF to the samples. The cells were then fixed, stained with crystal violet and photographed. Scale bar: 100 µm.