Fig. 8. The role of PLC-1 in E-lam formation. (A) To study the role of PLC- in E-lam formation, the spread cells were treated with PLC inhibitor U-71322 (0-4 µM) in the presence or in absence of staurosporine and analyzed for cell spreading and E-lam formation. The results show the mean±s.d. of one typical experiment (n=8). (*P<0.05; ^**P<0.01; ^***P<0.001.) (B) Immunolocalization of tyrosine phosphorylated (active) PLC-1 (a,b) and actin (c,d) in control (a,c) and staurosporine-treated (b,d) cells. PLC-1 and actin accumulated in E-lams are marked with arrows and arrowheads, respectively. Scale bar: 10 µm.