Fig. 3. Cell morphology under 2.5 and 0.5% agarose. Images of cells under agarose were acquired 5 hours after wild-type cells were added to the trough and 2 hours after mhcA^- cells were added. The trough edge interferes with the imaging, so the optical field was place about 300 µm from the edge of the trough. AX2 (wild type), ELC- (essential light chain mutant), ELC+ (essential light chain mutant rescued by expression of essential light chain cDNA), and HK321 (myosin heavy chain null mutants).