Fig. 4. Fragmentation of mhcA^- cells moving under agarose. Myosin mutant cells were imaged under 0.5% agarose, within 500 µm of trough edge. The origin of the gradient and direction of cell movement is indicated by the white arrow. The montage shows images of a single cell over time as it becomes stretched and fragmented. The black arrow indicates a cell fragment left behind. Numbers indicate time in seconds. A QuickTime movie showing the fragmentation events can be viewed at http://jcs.biologists.org/supplemental/.