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Fig. 5. GFP-ABD120 localization in mhcA- cells under agarose. Confocal images taken 100 seconds apart of GFP-ABD120 localization in mhcA- cell. The cell is moving in the direction indicated by the arrow. GFP-ABD localizes to F-actin primarily in the rear of the cell. Magnification is 63x with a z-section thickness of 0.5 µm. A QuickTime movie showing the dynamic localization can be viewed at http://jcs.biologists.org/supplemental/.