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Fig. 7. Localization of F-actin and myosin II during under-agarose migration. Confocal images taken of cells moving in the direction of the arrow under 2.0% agarose. The images shown are at 30-40 second intervals in a focal plane a few microns above the substratum. (A) Wild-type cells expressing GFP-myosin II. (B) mlcE- cells expressing GFP-Myosin II. (C) Wild-type cells expressing GFP-ABD120 to visualize F-actin dynamics. (D) mlcE- cells expressing GFP-ABD120. The localization and dynamics of actin and myosin II are not significantly altered in mlcE- cells. QuickTime movies of the fluorescence localization can be viewed at http://jcs.biologists.org/supplemental/.