Fig. 8. Three-dimensional localization of myosin II in cells moving under agarose. z-series through the cells were acquired with a confocal microscope while the cells were moving under agarose. The sections shown are spaced approximately 0.2 µm apart. (A) Wild-type cells expressing myosin-GFP. (B) mlcE^- cells expressing myosin-GFP. In both cases, the edge of the cell contains myosin-GFP throughout much of the 4-5 µm thickness. This would form a vertical wall of myosin and actin at the edge. The extent of this wall from front to back of the cell varies from cell to cell and over time. In many cells, the wall is primarily in an arc around the posterior of the cell as shown in Fig. 7A,B.