Fig. 1. Nascent inclusions of LGV and non-LGV serovars of C. trachomatis localize in the vicinity of the MTOC. Cos-7 cells were infected with C. trachomatis serovar L2 (LGV) or serovar D (non-LGV) chlamydial EBs at an MOI of 50. Cells were fixed 5 hours postinfection and stained via indirect immunofluorescence simultaneously with anti-L2 EB (red) and anti-ß-tubulin antibodies (green). The fluorescent staining revealed that nascent inclusions of either serovar aggregated at a single site corresponding to the MTOC (arrows). In dividing cells in which the MTOC has been duplicated, nascent inclusions were observed to localize at both spindle poles (arrowheads). Bar, 10 µM.