Fig. 2. Defective chemotactic response by cells transfected with the C-terminal deletion mutants. Mouse aortic endothelial cells (MAE) transfected with vector (Vec), angiomotin (Amot) or deletion mutants (1-4) were stimulated by addition of (A) 20 ng/ml bFGF, (B) 10 ng/ml VEGF or (C) 200 ng/ml LPA. The differences between angiomotin and the deletion mutants were statistically significant (asterisks denotes P<0.01, error bars=s.d.). Cell migration was stimulated by the addition the chemo-attractant to the lower chamber in a modified Boyden chamber. The number of cells migrating through a collagen 1-coated filter was estimated after 5 hours. All assays were performed in quadruplicate.