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Fig. 5. Expression of wild-type and {Delta}4-angiomotin in endothelial cells of transgenic mice. (A) TIE-angiomotin and TIE-{Delta}4 angiomotin constructs. (B) Southern blot showing integration of TIE constructs in angiomotin (TIE-amot) and {Delta}4-angiomotin (TIE-{Delta}4) transgenic embryos (E9.5) and adult mice, and expression of the transgenes, analyzed by RT-PCR, showing angiomotin expression in angiomotin (TIE-ang) and {Delta}4-angiomotin (TIE-{Delta}4) in transgenic embryos (E9.5) and adult mice (hAmot, human angiomotin). (C-H) Phenotype of TIE-angiomotin and TIE-{Delta}4 angiomotin embryos at E9.5. TIE-angiomotin embryos (D) are phenotypically indistinguishable from their wild-type littermates (C). (E) Wild-type littermate of the TIE-{Delta}4 angiomotin embryos. (F) Cranial hemorrhage in TIE-{Delta}4 angiomotin-expressing embryo. (G,H) Embryonic malformations in TIE-{Delta}4 angiomotin embryos.